The Association of Early Pregnancy Loss with Polymorphism in Xenobiotics Detoxification Genes

Abstract

Pregnancy loss and other pregnancy complication can be connected with environmental and lifestyle risk factor, among which effect of chemical compounds is the strongest. Effects of xenobiotics can be modified by allele variants of xenobiotic detoxification enzymes phase I or II. A total of 71 women with early pregnancy loss and 101 control patients were examined by a case-control methodology. The Ile462Val CYP1A1, Arg47His ADH1B, Glu487Lys ALDH, I105V GSTP1 polymorphisms were genotyped by allele-specific polymerase chain reaction. Our data demonstrated that the heterozygous Glu487Lys ALDH genotypes rate were higher in the pregnancy loss patients (12.71%) compared to the control group (2.0%). There was no difference between two groups detected for other polymorphisms. However, presence of polymorphic variants of genes of 1st and 2nd detoxification phases can have additive effect and cause multifactorial pathology risk increase. It is shown, that combination of polymorphic variants of ALDH2 И¸ GSTP1 genes in genotype results in 5 fold pregnancy loss risk increase. Combination of polymorphic variants of ALDH2, ADH1B u GSTP1 genes in genotype results in 9 fold pregnancy loss risk increase. The results demonstrated that combination of allele variants of 1st and 2nd detoxification phases in woman genotype increases the risk of early pregnancy loss.