Radiographic Evaluations of the Tetra-Calcium Phosphate and Diacalcium Phosphate with Bone Plate in Osseo-Integration of Bone Repair in Rabbit

Abstract

Problem statement: To determine the effect of the Tetra calcium phosphate and Diacalcium phosphate cement in comparison with bone plate in fracture repair in rabbit. Experimental in vivo study. A total of 18 adult male White New Zealand rabbits, weighing 3.0±0.250 Kg and about 6 months old were used. They were fasted 4 h before start of experiment and were off-water even 2 h too. They were divided into 2 groups of bone plate (I) and cement (II) of 9 rabbits each which subsequently divided into 3 subgroups of 1(I a and II a) month and 2 (Ib and IIb)and 3 (Ic and IIc) months duration in each group. Approach: Under general anesthesia and aseptic condition the craniolateral surface of right radial was exposed and a piece of full thickness from mid-section of radial bone in length of 1cm was removed using electrical bone vibrator in the all rabbit. The both ends of this bone was stabilized using mini bone plate of 4 holes in the group I , whereas prehardened cylinders of cement were implanted into rabbit radial defects in the group II rabbits. Radiographs were taken on zero days after creation of gap and on 1, 2 and 3 months from each rabbit using parameters such as local changes, stabilization of implant and bone, bone formation and implant biodegradation. Results: Comparison of clinical signs in the I and II indicated that they had similar physical activity and same degree of lameness during observation period of 90 days Radiographyically keeping in view the parameters were used to evaluate the major changes revealed no sign of infection on the edges of each defect and both groups permitted stabilization of the defected bones and maintenance of correct position. The new bone formation and the implant biodegradation in the bone defects could be clearly reflected by the gradually weakened umbral on the radiographs during the whole process one repair in group I was not as perfect as those in cement or group II samples. Conclusion/Recommendations: Application of paste of tetra calcium phosphate and diacalcium phosphate indicated to have positive effect in integral formation of qualitative callus at the site of fracture and early re-organization of callus too.