Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria
- 1 University of Batna 1, Batna, Algeria
- 2 High National Veterinary School, Algiers, Algeria
- 3 Laboratory of Molecular Biology and Microbiology of Constantine Biotechnology Research Center, Algeria
Abstract
The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection of the hlyA gene in all samples was done by real-time PCR assay by comparative cycle threshold method. The results obtained were negative for all the samples analyzed, therefore the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%. The absence of L. monocytogenes in the samples could be linked to the good health of the selected cattle. In fact, L. monocytogenes is considered to be very widespread in industrialized countries and rare in North Africa and Algeria.
DOI: https://doi.org/10.3844/ajavsp.2021.212.216
Copyright: © 2021 Sabrina Rabehi, Bakir Mamache, Taha Mossadak Hamdi, Asma Meghezzi and Khaoula Boushaba. This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Listeria monocytogenes
- Real-Time PCR
- Raw Milk
- Public Health