Lack of Association of Glutathione S-transferase Gene Polymorphisms in Iranian Prostate Cancer Subjects

Abstract

Problem statement: Glutathione S-transferase is super family genes that encode enzymes which involve in the detoxification of the cell and protects DNA from damage. Conflicting results was found in several studies in association with GSTT1 and GSTM1 gene polymorphisms and prostate cancer. In this study, we determined the association between the GSTT1 and GSTM1 gene polymorphism and prostate cancer in Iranian subjects as control with control subjects. Approach: Sixty number of sample were collected from both case and control at Milad hospital, Tehran, Iran who were above 40 years of old. Genomic DNA was extracted from blood and the detection of GSTT1 and GSTM1 genotypes was done using multiplex PCR method. Results: The frequency of the null alleles in GSTT1 and GSTM1 was 15 and 41.66% respectively in prostate cancer patients and 21.66 and 56.66% respectively in control subjects. There was no association was found between the polymorphism and prostate cancer among Iranian subjects (p>0.05). Conclusion: This study failed to show an association with GSTT1 and GSTM1 gene polymorphisms and prostate cancer and has no susceptibility to Iranian prostate cancer subjects.