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ISSN: 1553-3468 (Print)
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Research Article Open Access

Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)

Aimun Abdelgaffar Elhassan Ahmed1, Robert Gaspar1, Arpad Marki1, Andrea Vasas2, Mahmoud Mudawi Eltahir Mudawi3, Judit Hohmann3 and George Falkay1
  • 1 University of Szeged, Hungary
  • 2 Omdurman Islamic University, Sudan
  • 3 University of Szeged, Sudan

Abstract

Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its different fractions produced dose-dependent relaxant effect (p<0.05, t-test, n = 6) on uterine contraction elicit by 25 mM KCl in both NP and LP uteri that affected significantly by fractionation, however, the effect of the most active fraction (AH2-11) was reversed by prior addition of propranolol (non-specific β-antagonist), but not affected by progesterone pre treatment of the LP rats. In addition, AH2 only in high concentration displaced isotopes from β-ADR, this affinity changed markedly by fractionation. Conclusion: We validate the fractionation effect on its relaxant activity and found partial role for β-ADR on mediating this activity. Future study was recommended to isolate and investigate its active components to enhance this activity or to discover a new novel natural therapeutic agent(s).

American Journal of Biochemistry and Biotechnology
Volume 6 No. 3, 2010, 231-238

DOI: https://doi.org/10.3844/ajbbsp.2010.231.238

Submitted On: 4 November 2010 Published On: 30 September 2010

How to Cite: Ahmed, A. A. E., Gaspar, R., Marki, A., Vasas, A., Mudawi, M. M. E., Hohmann, J. & Falkay, G. (2010). Uterus-Relaxing Study of a Sudanese Herb (El-Hazha). American Journal of Biochemistry and Biotechnology, 6(3), 231-238. https://doi.org/10.3844/ajbbsp.2010.231.238

Open AccessCopyright: © 2010 Aimun Abdelgaffar Elhassan Ahmed, Robert Gaspar, Arpad Marki, Andrea Vasas, Mahmoud Mudawi Eltahir Mudawi, Judit Hohmann and George Falkay. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Haplophylium tuberculatum
  • radio-ligand binding assay
  • β-Adrenergic Receptors (β-ADR)
  • Non-Pregnant (NP)
  • Late-Pregnant rats (LP)
  • Sudanese plant
  • charles-river laboratories
  • medicinal and aromatic plants
  • tocolytic agent
  • pharmacological effects
  • progesterone treatment
  • uterus-relaxing study