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Frequency: Quarterly
ISSN: 1553-3468 (Print)
ISSN: 1558-6332 (Online)
Research Article Open Access

Production of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coli

Dessy Natalia1, Christian Heryakusuma1, Fernita Puspasari1, Linda Juniar1, Yovin Sugiyono1, Anita Yuwita2, Hofiya Djauhari3, Silvita F. Riswari3, Bachti Alisjahbana3 and Ihsanawati1
  • 1 Institut Teknologi Bandung, Indonesia
  • 2 Pakar Biomedika Indonesia, Indonesia
  • 3 Universitas Padjadjaran, Indonesia

Abstract

Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated and then inserted into an expression vector pET16b. As a hydrophobic protein with aliphatic index of 71.21%, DENV-2 NS1 was produced as inclusion bodies in E. coli BL21(DE3). The DENV-2 NS1 aggregate was unfolded in 8 M urea and then refolded by reverse dilution method. The refolded DENV-2 NS1 has immunologically active structure as it is capable to interact with anti-NS1 antibody, hence making it as a potential vaccine candidate.

American Journal of Biochemistry and Biotechnology
Volume 14 No. 3, 2018, 230-237

DOI: https://doi.org/10.3844/ajbbsp.2018.230.237

Submitted On: 3 July 2018 Published On: 10 October 2018

How to Cite: Natalia, D., Heryakusuma, C., Puspasari, F., Juniar, L., Sugiyono, Y., Yuwita, A., Djauhari, H., Riswari, S. F., Alisjahbana, B. & Ihsanawati, (2018). Production of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coli. American Journal of Biochemistry and Biotechnology, 14(3), 230-237. https://doi.org/10.3844/ajbbsp.2018.230.237

Open AccessCopyright: © 2018 Dessy Natalia, Christian Heryakusuma, Fernita Puspasari, Linda Juniar, Yovin Sugiyono, Anita Yuwita, Hofiya Djauhari, Silvita F. Riswari, Bachti Alisjahbana and Ihsanawati. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Dengue Virus Serotype 2
  • Non Structural Protein 1
  • Vaccine Candidate
  • Refolding
  • Escherichia coli