Study on the Synergistic Treatment of Soil Fumigation and Biocontrol Bacteria for the Prevention and Control of Root-Knot Nematodes in Facility Soil

Abstract

Years of continuous cropping have led to the inability to cure root-knot nematode disease. In this study, soil fumigation was used before tomato planting to study the decline rate and the effect of root-knot nematode population control after soil fumigation. During the experiment, "Wofengkang" organic sulfur soil fumigant was used. A constant temperature drying oven was used to artificially simulate the conventional greenhouse soil temperature to screen for optimal dosage of high-efficiency organic sulfur soil fumigant. The nematodes in each treatment were 0 and the reduction rate was 100% at the temperatures of 44 and55℃. To treat soil root-knot nematodes effectively, a dosage of 300-450 kg/hm² is recommended depending on the severity of the disease and the safe dosage of chemical fumigants. Tomatoes were planted after fumigation to measure the incidence rate, disease index, and population number of root-knot nematodes and to calculate the decline rate of nematodes. According to field experiments, the incidence rate, disease level, and disease index of root-knot nematodes were 64.37,3 and 60.18%, respectively, before and after fumigation. After the combination of the two, the three indicators were all 0 and reached a significant difference from that before treatment. There was a significant decline of 97.26% in the number of root-knot nematodes in 100g soil after fumigation compared to before fumigation. This technology can effectively prevent and control the root-knot nematode disease in greenhouse tomatoes.